nf κb subunit beta  (Cusabio)

Journal: Oncology reports

Article Title: KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

doi: 10.3892/or.2018.6687

Figure Lengend Snippet: Figure 5. (A) Western blotting of p-IκB-α, p-IKK-β and nuclear NF-κB p65 in SW579 cells (SW579/scrRNA, SW579/siKLF5#1 and SW579/siKLF5#2, left panel) and B-CPAP cells (B-CPAP/NC and B-CPAP/KLF5, right panel); and p84 was used as a nuclear marker. (B) Quantified western blotting results. (C) Immunofluorescent staining of NF-κB p65 in SW579/scrRNA, SW579/siKLF5#1, SW579/siKLF5#2, B-CPAP/NC and B-CPAP/KLF5 cells. NF-κB, nuclear factor-κB; p-, phosphorylated-; IKK-β, inhibitor of nuclear factor κB kinase subunit β; IκB-α, nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, α; scrRNA, scrambled RNA; si, small interfering RNA; NC, negative control; KLF5, Kruppel-like factor 5.

Article Snippet: The membranes were blocked with 5% bovine serum albumin (1 h at room temperature) and incubated with the corresponding primary antibodies including KLF5 rabbit polyclonal antibody (dilution 1:1,000; cat. no. 21017-1-AP; ProteinTech Group., Inc.), FBW7 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab74054; Abcam), nuclear matrix protein p84 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab487; Abcam), fibronectin rabbit polyclonal antibody (dilution 1:1,000; cat. no. ab2413; Abcam), E-Cadherin rabbit mAb (dilution 1:1,000; cat. no. 3195; Cell Signaling Technology, Inc.), Vimentin rabbit mAb (dilution 1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), Twist family BHLH transcription factor 1 (Twist1) rabbit mAb (dilution 1:1,000; cat. no. 46702; Cell Signaling Technology, Inc.), IκBα Mouse mAb (dilution 1:1,000; cat. no. 4814; Cell Signaling Technology, Inc.), phosphorylated (p)-IκBα rabbit mAb (dilution 1:1,000; cat. no. 2859; Cell Signaling Technology, Inc.), inhibitor of nuclear factor NF-κB subunit β (IKKβ) rabbit mAb (dilution 1:1,000; cat. no. 8943; Cell Signaling Technology, Inc.), p-IKKβ rabbit mAb (dilution 1:1,000; cat. no. 2697; Cell Signaling Technology, Inc.), NF-κB p65 rabbit mAb (dilution 1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p44/42 extracellular signal-regulated kinase 1 and 2 (Erk1/2) rabbit mAb (dilution 1:1,000; cat. no. 4695; Cell Signaling Technology, Inc.), p-p44/42 Erk1/2 rabbit mAb (dilution 1:1,000; cat. no. 4370; Cell Signaling Technology, Inc.), AKT rabbit mAb (dilution 1:1,000; cat. no. 4685; Cell Signaling Technology, Inc.), p-AKT rabbit mAb (dilution 1:1,000; cat. no. 4060; Cell Signaling Technology, Inc.) and GAPDH mouse monoclonal antibody (dilution 1:5,000; cat. no. 60004-1-Ig; ProteinTech Group., Inc.) at 4 ̊C overnight.

Techniques: Western Blot, Marker, Staining, Small Interfering RNA, Negative Control

Journal: Oncology reports

Article Title: KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

doi: 10.3892/or.2018.6687

Figure Lengend Snippet: Figure 6. KLF5 promoted cell growth and metastasis through the NF-κB signalling pathway. B-CPAP/KLF5 cells were treated with the specific NF-κB inhibitor SC75741 (5 µM) and the effect was assessed. (A) B-CPAP/KLF5 cells were treated with SC75741 or DMSO for 48 h. Western blotting of cell lysates from B-CPAP/NC, B-CPAP/KLF5, B-CPAP/KLF5+DMSO and B-CPAP/KLF5+SC75741 cells revealed that the NF-κB inhibitor SC75741 decreased the nuclear NF-κB p65 level of B-CPAP/KLF5 cells; and p84 was used as a nuclear marker. (B) Quantified western blotting results. NF-κB inhibitor SC75741 suppressed the (C) proliferation, (D) anchorage-independent growth and (E) migration/invasion ability of B-CPAP/KLF5 cells. NF-κB, nuclear factor-κB; NC, negative control; KLF5, Kruppel-like factor 5; DMSO, dimethyl sulfoxide.

Article Snippet: The membranes were blocked with 5% bovine serum albumin (1 h at room temperature) and incubated with the corresponding primary antibodies including KLF5 rabbit polyclonal antibody (dilution 1:1,000; cat. no. 21017-1-AP; ProteinTech Group., Inc.), FBW7 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab74054; Abcam), nuclear matrix protein p84 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab487; Abcam), fibronectin rabbit polyclonal antibody (dilution 1:1,000; cat. no. ab2413; Abcam), E-Cadherin rabbit mAb (dilution 1:1,000; cat. no. 3195; Cell Signaling Technology, Inc.), Vimentin rabbit mAb (dilution 1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), Twist family BHLH transcription factor 1 (Twist1) rabbit mAb (dilution 1:1,000; cat. no. 46702; Cell Signaling Technology, Inc.), IκBα Mouse mAb (dilution 1:1,000; cat. no. 4814; Cell Signaling Technology, Inc.), phosphorylated (p)-IκBα rabbit mAb (dilution 1:1,000; cat. no. 2859; Cell Signaling Technology, Inc.), inhibitor of nuclear factor NF-κB subunit β (IKKβ) rabbit mAb (dilution 1:1,000; cat. no. 8943; Cell Signaling Technology, Inc.), p-IKKβ rabbit mAb (dilution 1:1,000; cat. no. 2697; Cell Signaling Technology, Inc.), NF-κB p65 rabbit mAb (dilution 1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p44/42 extracellular signal-regulated kinase 1 and 2 (Erk1/2) rabbit mAb (dilution 1:1,000; cat. no. 4695; Cell Signaling Technology, Inc.), p-p44/42 Erk1/2 rabbit mAb (dilution 1:1,000; cat. no. 4370; Cell Signaling Technology, Inc.), AKT rabbit mAb (dilution 1:1,000; cat. no. 4685; Cell Signaling Technology, Inc.), p-AKT rabbit mAb (dilution 1:1,000; cat. no. 4060; Cell Signaling Technology, Inc.) and GAPDH mouse monoclonal antibody (dilution 1:5,000; cat. no. 60004-1-Ig; ProteinTech Group., Inc.) at 4 ̊C overnight.

Techniques: Western Blot, Marker, Migration, Negative Control

Journal: Oncology reports

Article Title: KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

doi: 10.3892/or.2018.6687

Figure Lengend Snippet: Figure 8. Correlation of KLF5 with the expression of FBW7 in the tissues of patients with thyroid cancer. (A) Representative IHC images of KLF5 and FBW7 in thyroid cancer tissues. (B) Correlation between the expression levels of KLF5 and FBW7 in thyroid cancer tissues. (C) Diagram presenting a summary of the results of the present study. It was proposed that the dysregulation of the tumor suppressor FBW7 reduces KLF5 degradation. Then, KLF5 promotes NF-κB p65 nuclear translocation, which in turn promotes the transcriptional levels of multiple downstream genes. FBW7, F-box/WD repeat-containing protein 7; KLF5, Kruppel-like factor 5; IHC, immunohistochemistry; NF-κB, nuclear factor-κB.

Article Snippet: The membranes were blocked with 5% bovine serum albumin (1 h at room temperature) and incubated with the corresponding primary antibodies including KLF5 rabbit polyclonal antibody (dilution 1:1,000; cat. no. 21017-1-AP; ProteinTech Group., Inc.), FBW7 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab74054; Abcam), nuclear matrix protein p84 mouse monoclonal antibody (dilution 1:1,000; cat. no. ab487; Abcam), fibronectin rabbit polyclonal antibody (dilution 1:1,000; cat. no. ab2413; Abcam), E-Cadherin rabbit mAb (dilution 1:1,000; cat. no. 3195; Cell Signaling Technology, Inc.), Vimentin rabbit mAb (dilution 1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), Twist family BHLH transcription factor 1 (Twist1) rabbit mAb (dilution 1:1,000; cat. no. 46702; Cell Signaling Technology, Inc.), IκBα Mouse mAb (dilution 1:1,000; cat. no. 4814; Cell Signaling Technology, Inc.), phosphorylated (p)-IκBα rabbit mAb (dilution 1:1,000; cat. no. 2859; Cell Signaling Technology, Inc.), inhibitor of nuclear factor NF-κB subunit β (IKKβ) rabbit mAb (dilution 1:1,000; cat. no. 8943; Cell Signaling Technology, Inc.), p-IKKβ rabbit mAb (dilution 1:1,000; cat. no. 2697; Cell Signaling Technology, Inc.), NF-κB p65 rabbit mAb (dilution 1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p44/42 extracellular signal-regulated kinase 1 and 2 (Erk1/2) rabbit mAb (dilution 1:1,000; cat. no. 4695; Cell Signaling Technology, Inc.), p-p44/42 Erk1/2 rabbit mAb (dilution 1:1,000; cat. no. 4370; Cell Signaling Technology, Inc.), AKT rabbit mAb (dilution 1:1,000; cat. no. 4685; Cell Signaling Technology, Inc.), p-AKT rabbit mAb (dilution 1:1,000; cat. no. 4060; Cell Signaling Technology, Inc.) and GAPDH mouse monoclonal antibody (dilution 1:5,000; cat. no. 60004-1-Ig; ProteinTech Group., Inc.) at 4 ̊C overnight.

Techniques: Expressing, Translocation Assay, Immunohistochemistry